Bovine Pancreatic prohormone, PPY ELISA KIT

Bovine Pancreatic prohormone, PPY ELISA Kit

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

PPY,Pancreatic prohormone,Pancreatic polypeptide,PP,PNP; pancreatic polypeptide Y; prepro-PP ,prepropancreatic polypeptide, Pancreatic prohormone percursor

Search name

Bovine PPY ELISA KIT ,Bovine Pancreatic prohormone ELISA KIT ,Bovine Pancreatic polypeptide ELISA KIT ,Bovine PP ELISA KIT ,Bovine PNP ELISA KIT ,Bovine pancreatic polypeptide Y ELISA KIT ,Bovine prepro-PP  ELISA KIT ,Bovine prepropancreatic polypeptide ELISA KIT ,Bovine Pancreatic prohormone precursor ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination of Bovine pancreatic prohormone,Pancreatic prohormone concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pancreatic prohormone. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Pancreatic prohormone and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Pancreatic prohormone, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Pancreatic prohormone in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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