Bovine Chromogranin- A, CHGA ELISA KIT

Bovine Chromogranin- A, ChgA ELISA Kit

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

Chromogranin-A(CgA), chromogranin A,CGA; SP-I; betagranin,N-terminal fragment of chromogranin; chromogranin-A; parathyroid secretory protein 1; pituitary secretory protein I; parathyroid secretory protein 1, CHGA

Search name

Bovine Chromogranin-A ELISA KIT ,Bovine CgA ELISA KIT ,Bovine chromogranin A ELISA KIT ,Bovine CGA ELISA KIT ,Bovine SP-I ELISA KIT ,Bovine betagranin ELISA KIT ,Bovine N-terminal fragment of chromogranin ELISA KIT ,Bovine chromogranin-A ELISA KIT ,Bovine parathyroid secretory protein 1 ELISA KIT ,Bovine pituitary secretory protein I ELISA KIT ,Bovine parathyroid secretory protein 1 ELISA KIT ,Bovine CHGA ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination of Bovine chromogranin-a,CgA concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to CgA. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for CgA and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain CgA, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of CgA in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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