Bovine Serum albumin, SA ELISA Kit 96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
Serum albumin, SA,albumin,serum albumin; PRO0883; PRO0903; PRO1341; 32 AA; albumin AA 34; cell growth inhibiting protein 42; growth-inhibiting protein 20,BSA
Search name
Bovine Serum albumin ELISA KIT ,Bovine SA ELISA KIT ,Bovine albumin ELISA KIT ,Bovine serum albumin ELISA KIT ,Bovine PRO0883 ELISA KIT ,Bovine PRO0903 ELISA KIT ,Bovine PRO1341 ELISA KIT ,Bovine 32 AA ELISA KIT ,Bovine albumin AA 34 ELISA KIT ,Bovine cell growth inhibiting protein 42 ELISA KIT ,Bovine growth-inhibiting protein 20 ELISA KIT ,Bovine BSA ELISA KIT, Bovine ALB ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of bovine Serum albumin concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Serum albumin. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Serum albumin and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Serum albumin, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Serum albumin in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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