Bovine Adiponectin, ADIPOQ ELISA KIT

Bovine adiponectin, ADIPOQ ELISA KIT
96 Tests
Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE
BEGINNING!

Synonyms
Adiponectin,Gelatin-binding protein,Adipose most abundant gene transcript 1 protein(apM-1),Adipocyte, C1q and collagen
domain-containing protein,30 kDa adipocyte complement-related protein,Adipocyte complement-related 30 kDa protein(ACRP30); ACDC;
ACRP30; ADIPQTL1; ADPN; APM-1; APM1; GBP28; adipose specific collagen-like factor; gelatin-binding protein 28

Search name
Bovine adiponectin ELISA Kit, Bovine ADP ELISA Kit, Bovine Adiponectin,Gelatin-binding protein ELISA Kit, Bovine Adipose
most abundant gene transcript 1 protein ELISA Kit, Bovine apM-1 ELISA Kit, Bovine Adipocyte ELISA Kit, Bovine C1q and
collagen domain-containing protein ELISA Kit, Bovine 30 kDa adipocyte complement-related protein ELISA Kit, Bovine
Adipocyte complement-related 30 kDa protein ELISA Kit, Bovine ACRP30 ELISA Kit, Bovine ACDC ELISA Kit, Bovine ADIPQTL1
ELISA Kit, Bovine ADPN ELISA Kit, Bovine APM1 ELISA Kit, Bovine GBP28 ELISA Kit, Bovine adipose specific collagen-like
factor ELISA Kit, Bovine gelatin-binding protein 28 ELISA Kit

Intended use
This immunoassay kit allows for the in vitro quantitative determination of Bovine adiponectin,Adiponectin concentrations in serum,
Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Adiponectin. Standards or samples are then
added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Adiponectin and Avidin
conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to
each well. Only those wells that contain Adiponectin, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in
color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured
spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Adiponectin in the samples is then determined by
comparing the O.D. of the samples to the standard curve.

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