Bovine Lactalbumin, LALBA ELISA Kit

Bovine Lactalbumin, LALBA ELISA Kit
96 Tests
Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE
BEGINNING!

Synonyms
Alpha-lactalbumin,Lactose synthase B protein,Lysozyme-like protein 7,LALBA,lactalbumin

Search name
Bovine Alpha-lactalbumin ELISA Kit, Bovine LALBA ELISA Kit, Bovine LALBA ELISA Kit, Bovine Lactose synthase B protein
ELISA Kit, Bovine Lysozyme-like protein 7 ELISA Kit, Bovine lactalbumin ELISA Kit

Intended use
This immunoassay kit allows for the in vitro quantitative determination of bovine alpha-lactalbumin,Alpha-lactalbumin concentrations in
serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Alpha-lactalbumin. Standards or samples are then
added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Alpha-lactalbumin and
Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is
added to each well. Only those wells that contain Alpha-lactalbumin, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit
a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is
measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Alpha-lactalbumin in the samples is then
determined by comparing the O.D. of the samples to the standard curve.

PDF manual download

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