Bovine Interleukin 1 Receptor antagonist ELISA Kit

Product Name:Bovine Interleukin 1 Receptor antagonist ELISA Kit
Packing:96T

Catalog No.:ELA-E0223b

Gene Name:Bovine Interleukin 1 Receptor antagonist

Detect Range:78-5000pg/ml

Sensitivity:46.9pg/ml

Target Protein Name:Bovine Interleukin 1 Receptor antagonist

Alternative Name:IL-1Ra,Bovine Interleukin 1 Receptor antagonist
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Bovine Interleukin 1 Receptor antagonist ELISA Kit allows for the in vitro quantitative determination of Bovine Interleukin 1 Receptor antagonist concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Bovine Interleukin 1 Receptor antagonist ELISA Kit has been pre-coated with an Bovine Interleukin 1 Receptor antagonist antibody specific to Bovine Interleukin 1 Receptor antagonist .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Bovine Interleukin 1 Receptor antagonist and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Bovine Interleukin 1 Receptor antagonist, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Bovine Interleukin 1 Receptor antagonist in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!

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