嘌呤霉素 来源于白色链球菌

Related Categories	Antibacterial, Antibiotics, Antibiotics A to Z,Antibiotics N-S, Antibiotics by Application, 更多...
Quality Level	PREMIUM
biological source	Streptomyces alboniger
InChI Key	MKSVFGKWZLUTTO-FZFAUISWSA-N
product line	BioReagent
assay	≥98% (HPLC)
form	powder
mol wt	544.43 g/mol
packaging	pkg of 10 mg
	pkg of 100 mg
	pkg of 25 mg
color	yellow-white
solubility	H2O: soluble 50 mg/mL, clear, colorless to faintly yellow
suitability	suitable for cell culture
Featured Industry	Agriculture
Mode of action	protein synthesis | interferes
UniProt accession no.	P13249
antibiotic activity spectrum	Gram-positive bacteria
	neoplastics
	parasites
storage temp.	−20°C

产品描述

General description

Chemical structure: peptidyl nucleoside

Puromycin is a broad spectrum antibiotic produced by Streptomyces alboniger. It is an analog of the 3′ end of aminoacyl-tRNA.^[5][6] It is an inhibitor of peptidyl transferase and is active in both prokaryotes and eukaryotes.^[6]

Application

Puromycin dihydrochloride from Streptomyces alboniger has been used:
• in the preparation of puromycin stock solution for puromycin sensitivity assay.^[1]
• to select lentivirus-infected cells containing puromycin resistant selection marker.^[3][4][2]

Puromycin dihydrochloride is a part of the amino-nucleoside family of antibiotics and is derived from Streptomyces alboniger. It is a broad spectrum antibiotic with antitumor activity, as an inhibitor of protein synthesis and has been used to study transcription regulatory mechanisms that control the sequential and coordinate expression of genes during cell differentiation. The recommended working concentration in eukaryotic cell culture is 1-10 μg/mL.

包装

10, 25, 100 mg in glass bottle

Biochem/physiol Actions

Puromycin associates in a non-specific manner with growing polypeptide chains, and thus, leads to premature termination of translation. It inhibits protein synthesis in two ways. It associates with the donor substrate, peptidyl-tRNA, in the P site and thus, functions as an acceptor substrate. Second, it competes with aminoacyl tRNA to bind with the A′ site within the peptidyl transferase center.^[6]

Nucleoside antibiotic; protein synthesis inhibitor; prevents growth of bacteria, protozoa, algae, and mammalian cells.

Caution

As supplied, this product remains active for four years when stored at -20°C.

Preparation Note

This product is soluble in water at 50 mg/mL. A stock solution should be prepared by filtration using a 0.22 μm filter and then stored in aliquots at -20°C. This product is also soluble in methanol at 10 mg/mL.

Other Notes

Keep container tightly closed in a dry and well-ventilated place.Keep in a dry place