Mouse Placenta growth factor,PlGF ELISA Kit
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
PlGF,PGF,PlGF-2; PLGF; D12S1900; PGFL; SHGC-10760; placenta growth factor; placental growth factor, vascular endothelial growth factor-related protein; placental growth factor
Search name
Mouse PlGF ELISA KIT ,Mouse PGF ELISA KIT ,Mouse PlGF-2 ELISA KIT ,Mouse PLGF ELISA KIT ,Mouse D12S1900 ELISA KIT ,Mouse PGFL ELISA KIT ,Mouse SHGC-10760 ELISA KIT ,Mouse placenta growth factor ELISA KIT ,Mouse placental growth factor ELISA KIT ,Mouse vascular endothelial growth factor-related protein ELISA KIT ,Mouse placental growth factor ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of mouse placenta growth factor,PlGF concentrations in serum, Plasma, Urine, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to PlGF. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for PlGF and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain PlGF, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of PlGF in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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