Mouse Pulmonary surfactant-associated protein C,SP-C ELISA Kit
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
Pulmonary surfactant-associated protein C,Pulmonary surfactant-associated proteolipid SPL(Val),SP5,SP-C,Pulmonary surfactant-associated proteolipid SPL(Val),SP5, SFTPC,BRICD6; PSP-C; SFTP2; SMDP2; SP-C; BRICHOS domain containing 6; SP5; pulmonary surfactant apoprotein-2 SP-C; pulmonary surfactant-associated protein C; pulmonary surfactant-associated proteolipid SPL(Val); surfactant protein C
Search name
Mouse Pulmonary surfactant-associated protein C ELISA KIT ,Mouse Pulmonary surfactant-associated proteolipid SPL(Val) ELISA KIT ,Mouse SP5 ELISA KIT ,Mouse SP-C ELISA KIT ,Mouse Pulmonary surfactant-associated proteolipid SPL(Val) ELISA KIT ,Mouse SP5 ELISA KIT ,Mouse SFTPC ELISA KIT ,Mouse BRICD6 ELISA KIT ,Mouse PSP-C ELISA KIT ,Mouse SFTP2 ELISA KIT ,Mouse SMDP2 ELISA KIT ,Mouse SP-C ELISA KIT ,Mouse BRICHOS domain containing 6 ELISA KIT ,Mouse SP5 ELISA KIT ,Mouse pulmonary surfactant apoprotein-2 SP-C ELISA KIT ,Mouse pulmonary surfactant-associated protein C ELISA KIT ,Mouse pulmonary surfactant-associated proteolipid SPL(Val) ELISA KIT ,Mouse surfactant protein C ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of mouse pulmonary surfactant-associated protein c,SP-C concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to SP-C. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for SP-C and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain SP-C, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of SP-C in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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