Mouse von Willebrand factor A domain-containing protein 8, VWA8 ELISA Kit
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
VWA8,KIAA0564; von Willebrand factor A domain-containing protein 8; von Willebrand factor A domain containing 8, Uncharacterized protein KIAA0564
Search name
Mouse VWA8 ELISA KIT ,Mouse KIAA0564 ELISA KIT ,Mouse von Willebrand factor A domain-containing protein 8 ELISA KIT ,Mouse von Willebrand factor A domain containing 8 ELISA KIT ,Mouse Uncharacterized protein KIAA0564 ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Mouse von Willebrand factor A domain-containing protein 8, VWA8 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to VWA8. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for VWA8 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain VWA8, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of VWA8 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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