Insulin,INS ELISA Kit/ Mouse Insulin,INS ELISA试剂盒

Mouse Insulin,INS ELISA Kit
96 Tests
Operating instructions

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!
PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms
Insulin,INS

Search name
Mouse Insulin ELISA Kit, Mouse INS ELISA Kit,Insulin ELISA Kit,INS ELISA Kit,ELISA Kit

Intended use
This immunoassay kit allows for the in vitro quantitative determination of mouse Insulin,INS concentrations in serum, plasma, tissue
homogenates, cell culture supernates, and other biological fluids.

Test principle
The ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been
pre-coated with an antibody specific to INS, During the reaction, INS in the sample or standard competes with a fixed amount of
biotin-labeled INS for sites on a pre-coated Monoclonal antibody specific to INS. Excess conjugate and unbound sample or standard are
washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a
TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and
the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of INS in the samples is then
determined by comparing the O.D. of the samples to the standard curve.

PDF manual download

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