Mouse Glutamine synthetase, GS ELISA Kit
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
Glutamine synthetase,GS,Glutamate decarboxylase,Glutamate--ammonia ligase,Glutamine synthetase,GLNS; PIG43; PIG59; cell proliferation-inducing protein 59; glutamate decarboxylase; glutamate--ammonia ligase; glutamine synthase; proliferation-inducing protein 43; glutamate-ammonia ligase,GLUL
Search name
Mouse Glutamine synthetase ELISA KIT ,Mouse GS ELISA KIT ,Mouse GLUL ELISA KIT, Mouse GLNS ELISA KIT ,Mouse PIG43 ELISA KIT ,Mouse PIG59 ELISA KIT ,Mouse cell proliferation-inducing protein 59 ELISA KIT ,Mouse glutamate decarboxylase ELISA KIT ,Mouse glutamate--ammonia ligase ELISA KIT,Mouse Glutamate decarboxylase ELISA KIT ,Mouse Glutamate--ammonia ligase ELISA KIT ,Mouse Glutamine synthetase ELISA KIT ,Mouse GLNS ELISA KIT ,Mouse PIG43 ELISA KIT ,Mouse PIG59 ELISA KIT ,Mouse cell proliferation-inducing protein 59 ELISA KIT ,Mouse glutamate decarboxylase ELISA KIT ,Mouse glutamate--ammonia ligase ELISA KIT ,Mouse glutamine synthase ELISA KIT ,Mouse proliferation-inducing protein 43 ELISA KIT ,Mouse glutamate-ammonia ligase ELISA KIT,Mouse GLUL ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Mouse glutamine synthetase,GS concentrations in serum, Plasma, Urine, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to GS. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for GS and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain GS, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of GS in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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