Endocrine gland vascular endothelial growth factor, EG-VE/ Mouse Endocrine gland vascular endothelial growth factor, EG-VE

Mouse Endocrine gland vascular endothelial growth factor, EG- VEGF ELISA Kit
96 Tests
Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE
BEGINNING!

Synonyms
Prokineticin-1,Endocrine-gland-derived vascular endothelial growth factor(EG-VEGF), PROK1,EG-VEGF; EGVEGF; PK1; PRK1; black
mamba toxin-related protein; endocrine-gland-derived vascular endothelial growth factor; mambakine; prokineticin-1; prokineticin 1

Search name
Mouse Prokineticin-1 ELISA Kit, Mouse Endocrine-gland-derived vascular endothelial growth factor ELISA Kit, Mouse EG-VEGF
ELISA Kit, Mouse PROK1 ELISA Kit, Mouse EGVEGF ELISA Kit, Mouse PK1 ELISA Kit, Mouse PRK1 ELISA Kit, Mouse black
mamba toxin-related protein ELISA Kit, Mouse mambakine ELISA Kit, Mouse Prokineticin 1 ELISA Kit

Intended use
This immunoassay kit allows for the in vitro quantitative determination of mouse EG-VEGF,EG-VEGF concentrations in serum, Plasma,
tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to EG-VEGF. Standards or samples are then added
to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for EG-VEGF and Avidin
conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to
each well. Only those wells that contain EG-VEGF, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in
color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured
spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of EG-VEGF in the samples is then determined by
comparing the O.D. of the samples to the standard curve.

PDF manual download

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