High mobility group protein B1, HMGB1 ELISA Kit/ Mouse High mobility group protein B1, HMGB1 ELISA试剂盒

Mouse High mobility group protein B1, HMGB1 ELISA Kit
96 Tests
Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE
BEGINNING!

Synonyms
High mobility group protein B1,;High mobility group protein 1(HMG-1),; HMGB1,high mobility group protein B1; HMG1; HMG3; SBP-1;
Amphoterin; HMG-1; Sulfoglucuronyl carbohydrate binding protein; high mobility group protein 1; high-mobility group (nonhistone
chromosomal) protein 1; high-mobility group box 1; high mobility group box 1

Search name
Mouse HMGB1 ELISA Kit,Mouse High mobility group protein B1 ELISA Kit,  Mouse High mobility group protein 1 ELISA Kit,Mouse HMG-1 ELISA Kit, Mouse HMG3 ELISA Kit, Mouse SBP-1 ELISA Kit, Mouse Amphoterin ELISA Kit, Mouse HMG-1 ELISA Kit, Mouse Sulfoglucuronyl carbohydrate binding protein ELISA Kit, Mouse nonhistone chromosomal protein 1 ELISA Kit,Mouse high-mobility group box 1 ELISA Kit, Mouse high mobility group box 1 ELISA Kit

Intended use
Mouse HMGB1 ELISA Kit allows for the in vitro quantitative determination of mouse high mobility group protein b1 concentrations in serum,
Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle
The microtiter plate provided in Mouse HMGB1 ELISA Kit has been pre-coated with an antibody specific to High mobility group protein B1. Standards or
samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for High
mobility group protein B1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then
a TMB substrate solution is added to each well. Only those wells that contain High mobility group protein B1, biotin-conjugated antibody
and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric
acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of High
mobility group protein B1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Mouse HMGB1 ELISA Kit PDF manual download

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