Mouse RTN1 (Reticulon- 1) ELISA Kit (CUSTOM)
96 Tests
Operating instructions
FOR RESEARCH USE ONLY!
Alternative name
Mouse Rtn1 ELISA KIT ,Mouse NSP ELISA KIT ,Mouse neuroendocrine-specific protein ELISA KIT ,Mouse reticulon 1 ELISA KIT
Intended use
Mouse RTN1 ELISA kit allows for the in vitro quantitative determination of Mouse Reticulon- 1 concentrations in serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.
Test principle
Mouse RTN1 ELISA kit is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to RTN1, During the reaction, RTN1 in the sample or standard competes with a fixed amount of biotin-labeled RTN1 for sites on a pre-coated Monoclonal antibody specific to RTN1. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of RTN1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Customized product Min.Order 5kits
Delivery time 4-6month
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