Heparin cofactor II ELISA Kit/ Mouse Heparin cofactor II ELISA试剂盒

96 Tests

Operating instruction

FOR RESEARCH USE ONLY!

Alternative name

Mouse heparin cofactor II ELISA KIT ,Mouse Heparin cofactor 2 ELISA KIT ,Mouse SERPIND1 ELISA KIT ,Mouse HCF2 ELISA KIT ,Mouse HCF-2 ELISA KIT ,Mouse C2 factor ELISA KIT ,Mouse host cell factor C2 ELISA KIT ,Mouse Serpin D1 ELISA KIT ,Mouse  HC-II;Protease inhibitor leuserpin-2 ELISA KIT ,Mouse D22S673 ELISA KIT ,Mouse HC2 ELISA KIT ,Mouse HCF2 ELISA KIT ,Mouse HCII ELISA KIT ,Mouse HLS2 ELISA KIT ,Mouse LS2 ELISA KIT ,Mouse THPH10 ELISA KIT ,Mouse leuserpin 2 ELISA KIT ,Mouse protease inhibitor leuserpin-2 ELISA KIT ,Mouse serine proteinase inhibitor clade Dmember 1 ELISA KIT ,Mouse serpin D1 ELISA KIT ,Mouse serpin peptidase inhibitor clade D member 1 ELISA KIT ,Mouse serpin family D member 1 ELISA KIT

Intended use

Mouse Heparin cofactor II ELISA Kit allows for the in vitro quantitative determination of mouse Heparin cofactor II concentrations in serum, Plasma, Urine,tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in Mouse Heparin cofactor II ELISA Kit has been pre-coated with an antibody specific to Heparin cofactor II. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Heparin cofactor II and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Heparin cofactor II, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Heparin cofactor II in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Mouse Heparin cofactor II manual request

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