Mouse Aspartate aminotransferase, GOT/ ASAT ELISA Kit
96 Tests
FOR RESEARCH USE ONLY!
Alternative name
Mouse ASAT ELISA KIT ,Mouse Aspartate aminotransferase ELISA KIT ,Mouse GOT1 ELISA KIT ,Mouse GOT ELISA KIT ,Mouse aspartate aminotransferase 1 ELISA KIT ,Mouse AST1 ELISA KIT ,Mouse ASTQTL1 ELISA KIT ,Mouse GIG18 ELISA KIT ,Mouse cAspAT ELISA KIT ,Mouse cCAT ELISA KIT ,Mouse aspartate transaminase 1 ELISA KIT ,Mouse cysteine aminotransferase ELISA KIT ,Mouse cytoplasmic ELISA KIT ,Mouse cysteine transaminase cytoplasmic ELISA KIT ,Mouse glutamate oxaloacetate transaminase 1 ELISA KIT ,Mouse growth-inhibiting protein 18 ELISA KIT ,Mouse transaminase A ELISA KIT ,Mouse soluble glutamic-oxaloacetic transaminase 1 ELISA KIT ,Mouse Aspartate aminotransferase cytoplasmic ELISA KIT
Intended use
Mouse ASAT ELISA KIT allows for the in vitro quantitative determination of mouse Aspartate aminotransferase cytoplasmic concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Aspartate aminotransferase, cytoplasmic. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Aspartate aminotransferase, cytoplasmic and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Aspartate aminotransferase, cytoplasmic, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Aspartate aminotransferase, cytoplasmic in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Range 1.56 -100U/L
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