Mouse Synaptosomal-associated protein 25,SNAP25 ELISA Kit
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE
BEGINNING!
Synonyms
Synaptosomal-associated protein 25,SNAP-25,Super protein,SUP,Synaptosomal-associated 25 kDa protein,SNAP,super protein; RIC-4; RIC4; SEC9; SNAP-25; bA416N4.2; dJ1068F16.2; SUP; resistance to inhibitors of cholinesterase 4 homolog; synaptosomal-associated protein 25; synaptosomal-associated protein 25kDa
Search name
Mouse Synaptosomal-associated protein 25 ELISA KIT ,Mouse SNAP-25 ELISA KIT ,Mouse Super protein ELISA KIT ,Mouse SUP ELISA KIT ,Mouse Synaptosomal-associated 25 kDa protein ELISA KIT ,Mouse SNAP ELISA KIT ,Mouse super protein ELISA KIT ,Mouse RIC-4 ELISA KIT ,Mouse RIC4 ELISA KIT ,Mouse SEC9 ELISA KIT ,Mouse SNAP-25 ELISA KIT ,Mouse bA416N4.2 ELISA KIT ,Mouse dJ1068F16.2 ELISA KIT ,Mouse SUP ELISA KIT ,Mouse resistance to inhibitors of cholinesterase 4 homolog ELISA KIT ,Mouse synaptosomal-associated protein 25 ELISA KIT ,Mouse synaptosomal-associated protein 25kDa ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Mouse synaptosomal-associated protein 25,SNAP-25 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to SNAP-25. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for SNAP-25 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain SNAP-25, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of SNAP-25 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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