Cyclin-dependent kinase 4 inhibitor B, Cdkn2b ELISA KIT/ Mouse Cyclin-dependent kinase 4 inhibitor B, Cdkn2b ELISA试剂盒

Product Name:Mouse Cyclin- dependent kinase 4 inhibitor B, Cdkn2b ELISA KIT
Packing:96T

Catalog No.:ELI-50298m

Gene Name:Mouse Cdkn2b

Detect Range:78.1-5000pg/ml

Sensitivity:46.875pg/ml

Target Protein Name:Mouse Cdkn2b

Alternative Name:Mouse Cyclin-dependent kinase 4 inhibitor B, Cdkn2b
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Mouse Cyclin- dependent kinase 4 inhibitor B, Cdkn2b ELISA KIT allows for the in vitro quantitative determination of Mouse Cdkn2b concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Mouse Cyclin- dependent kinase 4 inhibitor B, Cdkn2b ELISA KIT has been pre-coated with an Mouse Cyclin-dependent kinase 4 inhibitor B, Cdkn2b antibody specific to Mouse Cdkn2b .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Cdkn2b and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Cdkn2b , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Cdkn2b in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!

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