ACOT6 (acyl-coenzyme A thioesterase 6) ELISA Kit/ Mouse ACOT6 (acyl-coenzyme A thioesterase 6) ELISA试剂盒

Mouse ACOT6 (acyl- coenzyme A thioesterase 6) ELISA Kit

96 Tests

Operating instructions

FOR RESEARCH USE ONLY!

Alternative name

Mouse ACOT6 ELISA KIT ,Mouse C14orf42 ELISA KIT ,Mouse c14_5530 ELISA KIT ,Mouse acyl-CoA thioesterase 6 ELISA KIT

Intended use

Mouse ACOT6 ELISA kit allows for the in vitro quantitative determination of Mouse acyl- coenzyme A thioesterase 6 concentrations in serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.

Test principle

Mouse ACOT6 ELISA kit is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to ACOT6, During the reaction, ACOT6 in the sample or standard competes with a fixed amount of biotin-labeled ACOT6 for sites on a pre-coated Monoclonal antibody specific to ACOT6. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of ACOT6 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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