Copper-transporting ATPase 1, Atp7a ELISA KIT/ Mouse Copper-transporting ATPase 1, Atp7a ELISA试剂盒

Mouse Copper- transporting ATPase 1, ATP7A ELISA KIT

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

Class 3.6.3.54,Copper-transporting ATPase 1,Copper pump 1,Menkes disease-associated protein, ATP7A,copper-transporting ATPase 1; DSMAX; MNK; SMAX3; Cu++-transporting P-type ATPase; Menkes disease-associated protein; copper pump 1; ATPase, Cu++ transporting alpha polypeptide, MC1,MNK,

Search name

Mouse Class 3.6.3.54 ELISA KIT ,Mouse Copper-transporting ATPase 1 ELISA KIT ,Mouse Copper pump 1 ELISA KIT ,Mouse Menkes disease-associated protein ELISA KIT ,Mouse  ATP7A ELISA KIT ,Mouse copper-transporting ATPase 1 ELISA KIT ,Mouse DSMAX ELISA KIT ,Mouse MNK ELISA KIT ,Mouse SMAX3 ELISA KIT ,Mouse Cu++-transporting P-type ATPase ELISA KIT ,Mouse Menkes disease-associated protein ELISA KIT ,Mouse copper pump 1 ELISA KIT ,Mouse ATPase ELISA KIT ,Mouse Cu++ transporting alpha polypeptide ELISA KIT ,Mouse MC1 ELISA KIT ,Mouse MNK ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination of Mouse Copper-transporting ATPase 1 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to Copper-transporting ATPase 1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Copper-transporting ATPase 1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Copper-transporting ATPase 1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Copper-transporting ATPase 1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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