Mouse ICOS ligand, ICOSLG ELISA KIT
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
ICOS ligand,LICOS,B7-related protein 1(B7RP-1),B7 homolog 2(B7-H2),B7-like protein Gl50,ICOSLG,B7-H2; B7H2; B7RP-1; B7RP1; CD275; GL50; ICOS-L; ICOSL; LICOS; B7 homolog 2; B7 homologue 2; B7-like protein Gl50; B7-related protein 1; ICOS ligand; transmembrane protein B7-H2 ICOS ligand; inducible T-cell co-stimulator ligand
Search name
Mouse ICOS ligand ELISA KIT ,Mouse LICOS ELISA KIT ,Mouse B7-related protein 1ELISA KIT ,Mouse B7 homolog 2(B7-H2) ELISA KIT ,Mouse B7-like protein Gl50 ELISA KIT ,Mouse ICOSLG ELISA KIT ,Mouse B7-H2 ELISA KIT ,Mouse B7H2 ELISA KIT ,Mouse B7RP-1 ELISA KIT ,Mouse B7RP1 ELISA KIT ,Mouse CD275 ELISA KIT ,Mouse GL50 ELISA KIT ,Mouse ICOS-L ELISA KIT ,Mouse ICOSL ELISA KIT ,Mouse LICOS ELISA KIT ,Mouse B7 homolog 2 ELISA KIT ,Mouse B7 homologue 2 ELISA KIT ,Mouse B7-like protein Gl50 ELISA KIT ,Mouse B7-related protein 1 ELISA KIT ,Mouse ICOS ligand ELISA KIT ,Mouse transmembrane protein B7-H2 ICOS ligand ELISA KIT ,Mouse inducible T-cell co-stimulator ligand ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of mouse ICOS ligand concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to ICOS ligand. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for ICOS ligand and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain ICOS ligand, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of ICOS ligand in the samples is then determined by comparing the O.D. of the samples to the standard curve.
PDF Manual download
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