Mouse Transmembrane glycoprotein NMB, GPNMB ELISA Kit
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
GPNMB,HGFIN; NMB; glycoprotein (transmembrane) nmb; glycoprotein nmb-like protein; glycoprotein nonmetastatic melanoma protein B; hematopoietic growth factor inducible neurokinin-1; osteoactivin; transmembrane glycoprotein HGFIN; glycoprotein nmb, Transmembrane glycoprotein NMB,Osteoactivin,DC-HIL,Dendritic cell-associated transmembrane protein
Search name
Mouse GPNMB ELISA KIT ,Mouse HGFIN ELISA KIT ,Mouse NMB ELISA KIT ,Mouse glycoprotein (transmembrane) nmb ELISA KIT ,Mouse glycoprotein nmb-like protein ELISA KIT ,Mouse glycoprotein nonmetastatic melanoma protein B ELISA KIT ,Mouse hematopoietic growth factor inducible neurokinin-1 ELISA KIT ,Mouse osteoactivin ELISA KIT ,Mouse transmembrane glycoprotein HGFIN ELISA KIT ,Mouse glycoprotein nmb ELISA KIT ,Mouse Transmembrane glycoprotein NMB ELISA KIT ,Mouse Osteoactivin ELISA KIT ,Mouse DC-HIL ELISA KIT ,Mouse Dendritic cell-associated transmembrane protein ELISA KIT
Intended use
Mouse GPNMB ELISA KIT allows for the in vitro quantitative determination of mouse transmembrane glycoprotein nmb,Transmembrane glycoprotein NMB concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in Mouse GPNMB ELISA KIT has been pre-coated with an antibody specific to Transmembrane glycoprotein NMB. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Transmembrane glycoprotein NMB and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Transmembrane glycoprotein NMB, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Transmembrane glycoprotein NMB in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Mouse GPNMB ELISA KIT Range 0.15-10ng/ml
Mouse GPNMB ELISA KIT PDF Manual download
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