Mouse MBNL1 (Muscleblind- like protein 1) ELISA Kit
96 Tests
Operating instructions
FOR RESEARCH USE ONLY!
Alternative name
Mouse MBNL1 ELISA KIT ,Mouse EXP ELISA KIT ,Mouse MBNL ELISA KIT ,Mouse triplet-expansion RNA-binding protein ELISA KIT ,Mouse muscleblind like splicing regulator 1 ELISA KIT
Intended use
Mouse MBNL1 ELISA kit allows for the in vitro quantitative determination of Mouse Muscleblind- like protein 1 concentrations in serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.
Test principle
Mouse MBNL1 ELISA kit is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to MBNL1, During the reaction, MBNL1 in the sample or standard competes with a fixed amount of biotin-labeled MBNL1 for sites on a pre-coated Monoclonal antibody specific to MBNL1. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of MBNL1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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