Mouse Iroquois- class homeodomain protein IRX- 4, Irx4 ELISA KIT Product Name:Mouse Iroquois- class homeodomain protein IRX- 4, Irx4 ELISA KIT
Packing:96T
Catalog No.:ELI-38553m
Gene Name:Mouse Irx4
Detect Range:15.6-1000pg/ml
Sensitivity:9.375pg/ml
Target Protein Name:Mouse Irx4
Alternative Name:Mouse Iroquois-class homeodomain protein IRX-4, Irx4
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Iroquois- class homeodomain protein IRX- 4, Irx4 ELISA KIT allows for the in vitro quantitative determination of Mouse Irx4 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Iroquois- class homeodomain protein IRX- 4, Irx4 ELISA KIT has been pre-coated with an Mouse Iroquois-class homeodomain protein IRX-4, Irx4 antibody specific to Mouse Irx4 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Irx4 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Irx4 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Irx4 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!

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