NFAT activation molecule 1, Nfam1 ELISA KIT/ Mouse NFAT activation molecule 1, Nfam1 ELISA试剂盒

Mouse NFAT activation molecule 1, NFAM1 ELISA Kit

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

NFAT activation molecule 1,Calcineurin/NFAT-activating ITAM-containing protein,NFAT-activating protein with ITAM motif 1, NFAM1,CNAIP,bK126B4.4; NFAT activation molecule 1; NFAT-activating protein with ITAM motif 1; calcinerin/NFAT-activating ITAM-containing protein; calcineurin/NFAT-activating ITAM-containing protein; NFAT activating protein with ITAM motif 1

Search name

Mouse NFAT activation molecule 1 ELISA KIT ,Mouse Calcineurin/NFAT-activating ITAM-containing protein ELISA KIT ,Mouse NFAT-activating protein with ITAM motif 1 ELISA KIT ,Mouse NFAM1 ELISA KIT ,Mouse CNAIP ELISA KIT ,Mouse bK126B4.4 ELISA KIT ,Mouse NFAT activation molecule 1 ELISA KIT ,Mouse NFAT-activating protein with ITAM motif 1 ELISA KIT ,Mouse calcinerin/NFAT-activating ITAM-containing protein ELISA KIT ,Mouse calcineurin/NFAT-activating ITAM-containing protein ELISA KIT ,Mouse NFAT activating protein with ITAM motif 1 ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination of mouse nfat activation molecule 1,NFAM1 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to NFAM1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for NFAM1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain NFAM1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of NFAM1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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