IMPG1 (Interphotoreceptor matrix proteoglycan 1) ELISA Kit/ Mouse IMPG1 (Interphotoreceptor matrix proteoglycan 1) ELISA试剂盒

Mouse IMPG1 (Interphotoreceptor matrix proteoglycan 1) ELISA Kit (CUSTOM)

96 Tests

Operating instructions

FOR RESEARCH USE ONLY!

Alternative name

Mouse IMPG1 ELISA KIT ,Mouse GP147 ELISA KIT ,Mouse IPM150 ELISA KIT ,Mouse SPACR ELISA KIT ,Mouse VMD4 ELISA KIT ,Mouse interphotoreceptor matrix proteoglycan of 150 kDa ELISA KIT ,Mouse sialoprotein associated with cones and rods ELISA KIT ,Mouse interphotoreceptor matrix proteoglycan 1 ELISA KIT

Intended use

Mouse IMPG1 ELISA kit allows for the in vitro quantitative determination of Mouse Interphotoreceptor matrix proteoglycan 1 concentrations in serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.

Test principle

Mouse IMPG1 ELISA kit is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to IMPG1, During the reaction, IMPG1 in the sample or standard competes with a fixed amount of biotin-labeled IMPG1 for sites on a pre-coated Monoclonal antibody specific to IMPG1. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of IMPG1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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