Phosphatidylinositol-glycan-specific phospholipase D, Gp/ Mouse Phosphatidylinositol-glycan-specific phospholipase D, Gp

Mouse Phosphatidylinositol- glycan- specific phospholipase D, GPLD1 ELISA KIT

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

Phosphatidylinositol-glycan-specific phospholipase D,PI-G PLD,Glycoprotein phospholipase D,Glycosyl-phosphatidylinositol-specific phospholipase D,GPI-PLD,GPI-specific phospholipase ,GPLD1,GPIPLD; GPIPLDM; PIGPLD; PIGPLD1; PLD; GPI-PLD; GPI-specific phospholipase D; PI-G PLD; glycoprotein phospholipase D; phosphatidylinositol-glycan-specific phospholipase D; glycosylphosphatidylinositol specific phospholipase D1

Search name

Mouse Phosphatidylinositol-glycan-specific phospholipase D ELISA KIT ,Mouse PI-G PLD ELISA KIT ,Mouse Glycoprotein phospholipase D ELISA KIT ,Mouse Glycosyl-phosphatidylinositol-specific phospholipase D ELISA KIT ,Mouse GPI-PLD ELISA KIT ,Mouse GPI-specific phospholipase  ELISA KIT ,Mouse GPLD1 ELISA KIT ,Mouse GPIPLD ELISA KIT ,Mouse GPIPLDM ELISA KIT ,Mouse PIGPLD ELISA KIT ,Mouse PIGPLD1 ELISA KIT ,Mouse PLD ELISA KIT ,Mouse GPI-PLD ELISA KIT ,Mouse GPI-specific phospholipase D ELISA KIT ,Mouse PI-G PLD ELISA KIT ,Mouse glycoprotein phospholipase D ELISA KIT ,Mouse phosphatidylinositol-glycan-specific phospholipase D ELISA KIT ,Mouse glycosylphosphatidylinositol specific phospholipase D1 ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination of Mouse phosphatidylinositol-glycan-specific phospholipase d,PI-G PLD concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to PI-G PLD. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for PI-G PLD and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain PI-G PLD, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of PI-G PLD in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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