Mouse APOBEC1 complementation factor, A1cf ELISA KIT Product Name:Mouse APOBEC1 complementation factor, A1cf ELISA KIT
Packing:96T
Catalog No.:ELI-35057m
Gene Name:Mouse A1cf
Detect Range:31.25-2000pg/ml
Sensitivity:18.75pg/ml
Target Protein Name:Mouse A1cf
Alternative Name:Mouse APOBEC1 complementation factor, A1cf
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse APOBEC1 complementation factor, A1cf ELISA KIT allows for the in vitro quantitative determination of Mouse A1cf concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse APOBEC1 complementation factor, A1cf ELISA KIT has been pre-coated with an Mouse APOBEC1 complementation factor, A1cf antibody specific to Mouse A1cf .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse A1cf and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse A1cf , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse A1cf in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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