Mouse Apoptosis- enhancing nuclease, Aen ELISA KIT Product Name:Mouse Apoptosis- enhancing nuclease, Aen ELISA KIT
Packing:96T
Catalog No.:ELI-34824m
Gene Name:Mouse Aen
Detect Range:78.1-5000pg/ml
Sensitivity:46.875pg/ml
Target Protein Name:Mouse Aen
Alternative Name:Mouse Apoptosis-enhancing nuclease, Aen
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Apoptosis- enhancing nuclease, Aen ELISA KIT allows for the in vitro quantitative determination of Mouse Aen concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Apoptosis- enhancing nuclease, Aen ELISA KIT has been pre-coated with an Mouse Apoptosis-enhancing nuclease, Aen antibody specific to Mouse Aen .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Aen and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Aen , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Aen in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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