Mouse Vesicle- associated membrane protein 2, Vamp2 ELISA KIT Product Name:Mouse Vesicle- associated membrane protein 2, Vamp2 ELISA KIT
Packing:96T
Catalog No.:ELI-35258m
Gene Name:Mouse Vamp2
Detect Range:78.1-5000pg/ml
Sensitivity:46.875pg/ml
Target Protein Name:Mouse Vamp2
Alternative Name:Mouse Vesicle-associated membrane protein 2, Vamp2
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Vesicle- associated membrane protein 2, Vamp2 ELISA KIT allows for the in vitro quantitative determination of Mouse Vamp2 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Vesicle- associated membrane protein 2, Vamp2 ELISA KIT has been pre-coated with an Mouse Vesicle-associated membrane protein 2, Vamp2 antibody specific to Mouse Vamp2 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Vamp2 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Vamp2 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Vamp2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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