Mouse 2- aminoethanethiol dioxygenase, Ado ELISA KIT Product Name:Mouse 2- aminoethanethiol dioxygenase, Ado ELISA KIT
Packing:96T
Catalog No.:ELI-35154m
Gene Name:Mouse Ado
Detect Range:15.62-1000pg/ml
Sensitivity:9.375pg/ml
Target Protein Name:Mouse Ado
Alternative Name:Mouse 2-aminoethanethiol dioxygenase, Ado
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse 2- aminoethanethiol dioxygenase, Ado ELISA KIT allows for the in vitro quantitative determination of Mouse Ado concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse 2- aminoethanethiol dioxygenase, Ado ELISA KIT has been pre-coated with an Mouse 2-aminoethanethiol dioxygenase, Ado antibody specific to Mouse Ado .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Ado and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Ado , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Ado in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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