ASAH2 (Neutral ceramidase) ELISA Kit (CUSTOM)/ Mouse ASAH2 (Neutral ceramidase) ELISA试剂盒 (CUSTOM)

Mouse ASAH2 (Neutral ceramidase) ELISA Kit (CUSTOM)

96 Tests

Operating instructions

FOR RESEARCH USE ONLY!

Alternative name

Mouse ASAH2 ELISA KIT ,Mouse BCDase ELISA KIT ,Mouse HNAC1 ELISA KIT ,Mouse LCDase ELISA KIT ,Mouse N-CDase ELISA KIT ,Mouse NCDase ELISA KIT ,Mouse N-acylsphingosine amidohydrolase 2 ELISA KIT ,Mouse acylsphingosine deacylase 2 ELISA KIT ,Mouse mitochondrial ceramidase ELISA KIT ,Mouse neutral/alkaline ceramidase ELISA KIT ,Mouse non-lysosomal ceramidase ELISA KIT ,Mouse N-acylsphingosine amidohydrolase (non-lysosomal ceramidase) 2 ELISA KIT

Intended use

Mouse ASAH2 ELISA kit allows for the in vitro quantitative determination of Mouse Neutral ceramidase concentrations in serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.

Test principle

Mouse ASAH2 ELISA kit is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to ASAH2, During the reaction, ASAH2 in the sample or standard competes with a fixed amount of biotin-labeled ASAH2 for sites on a pre-coated Monoclonal antibody specific to ASAH2. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of ASAH2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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