Bombesin receptor subtype-3, Brs3 ELISA KIT/ Mouse Bombesin receptor subtype-3, Brs3 ELISA试剂盒

Mouse Bombesin receptor subtype- 3, BRS3 ELISA Kit

96 Tests

Operating instructions

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

BRS3,BB3; BRS-3; G-protein coupled receptor; bombesin receptor subtype 3; bombesin receptor subtype-3; bombesin-like receptor 3

Search name

Mouse BRS3 ELISA KIT ,Mouse BB3 ELISA KIT ,Mouse BRS-3 ELISA KIT ,Mouse G-protein coupled receptor ELISA KIT ,Mouse bombesin receptor subtype 3 ELISA KIT ,Mouse bombesin receptor subtype-3 ELISA KIT ,Mouse bombesin-like receptor 3 ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination of Mouse Bombesin receptor subtype- 3, BRS3 concentrations in serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.

Test principle

The ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to BRS3, During the reaction, BRS3 in the sample or standard competes with a fixed amount of biotin-labeled BRS3 for sites on a pre-coated Monoclonal antibody specific to BRS3. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of BRS3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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