Putative ATP-dependent Clp protease proteolytic subunit, / Mouse Putative ATP-dependent Clp protease proteolytic subunit,

Mouse Putative ATP-dependent Clp protease proteolytic subunit, mitochondrial, CLPP ELISA Kit

96 Tests

Operating instructions

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

Class 3.4.21.92,Putative ATP-dependent Clp protease proteolytic subunit, mitochondrial),Endopeptidase Clp,CLPP,ClpP caseinolytic protease, ATP-dependent, proteolytic subunit homolog; ClpP caseinolytic peptidase, ATP-dependent, proteolytic subunit homolog; ClpP caseinolytic peptidase ATP-dependent, proteolytic subunit; PRLTS3; ATP-dependent protease ClpAP, proteolytic subunit, Mouse; endopeptidase Clp; caseinolytic mitochondrial matrix peptidase proteolytic subunit

Search name

Mouse CLPP ELISA KIT ,Mouse ClpP caseinolytic protease ELISA KIT ,Mouse  ATP-dependent ELISA KIT ,Mouse  proteolytic subunit homolog ELISA KIT ,Mouse ClpP caseinolytic peptidase ELISA KIT ,Mouse  ATP-dependent ELISA KIT ,Mouse  proteolytic subunit homolog ELISA KIT ,Mouse ClpP caseinolytic peptidase ATP-dependent ELISA KIT ,Mouse proteolytic subunit ELISA KIT ,Mouse PRLTS3 ELISA KIT ,Mouse ATP-dependent protease ClpAP ELISA KIT ,Mouse proteolytic subunit ELISA KIT , Mouse endopeptidase Clp ELISA KIT ,Mouse caseinolytic mitochondrial matrix peptidase proteolytic subunit ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination of Mouse putative atp-dependent clp protease proteolytic subunit, mitochondrial,CLPP concentrations in serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.

Test principle

The ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to CLPP, During the reaction, CLPP in the sample or standard competes with a fixed amount of biotin-labeled CLPP for sites on a pre-coated Monoclonal antibody specific to CLPP. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of CLPP in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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