Activation-induced cytidine deaminase, Aicda ELISA KIT/ Mouse Activation-induced cytidine deaminase, Aicda ELISA试剂盒

Mouse Activation- induced cytidine deaminase, AICDA ELISA Kit

96 Tests

Operating instructions

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

AICDA,activation induced cytidine deaminase,AID; ARP2; CDA2; HEL-S-284; HIGM2; cytidine aminohydrolase; epididymis secretory protein Li 284; integrated into Burkitt's lymphoma cell line Ramos; single-stranded DNA cytosine deaminase; activation-induced cytidine deaminase

Search name

Mouse AICDA ELISA KIT ,Mouse activation induced cytidine deaminase ELISA KIT ,Mouse AID ELISA KIT ,Mouse ARP2 ELISA KIT ,Mouse CDA2 ELISA KIT ,Mouse HEL-S-284 ELISA KIT ,Mouse HIGM2 ELISA KIT ,Mouse cytidine aminohydrolase ELISA KIT ,Mouse epididymis secretory protein Li 284 ELISA KIT ,Mouse integrated into Burkitt's lymphoma cell line Ramos ELISA KIT ,Mouse single-stranded DNA cytosine deaminase ELISA KIT ,Mouse activation-induced cytidine deaminase ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination of Mouse Activation- induced cytidine deaminase, AICDA concentrations in serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.

Test principle

The ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to AICDA, During the reaction, AICDA in the sample or standard competes with a fixed amount of biotin-labeled AICDA for sites on a pre-coated Monoclonal antibody specific to AICDA. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of AICDA in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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