Mouse Amine oxidase [flavin- containing] B, MAOB ELISA KIT
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
MAOB,MAO brain; MAO platelet; MAO-B; adrenalin oxidase; monoamine oxidase type B; tyramine oxidase; monoamine oxidase B, Amine oxidase [flavin-containing] B
Search name
Mouse MAOB ELISA KIT ,Mouse MAO brain ELISA KIT ,Mouse MAO platelet ELISA KIT ,Mouse MAO-B ELISA KIT ,Mouse adrenalin oxidase ELISA KIT ,Mouse monoamine oxidase type B ELISA KIT ,Mouse tyramine oxidase ELISA KIT ,Mouse monoamine oxidase B ELISA KIT ,Mouse Amine oxidase [flavin-containing] B ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of mouse amine oxidase [flavin-containing] b, MAOB concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to MAOB. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for MAOB and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain MAOB, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of MAOB in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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