Annexin A8, Anxa8 ELISA KIT/ Mouse Annexin A8, Anxa8 ELISA试剂盒

96 Tests

Alternative name

Intended use

Mouse ANXA8 ELISA kit allows for the in vitro quantitative determination of Mouse ANXA8 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

Mouse ANXA8 ELISA kit Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for ANXA8 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain ANXA8, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of ANXA8 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Mouse ANXA8 ELISA kit  Range 78-5000pg/ml

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