Ctf2 (Cardiotrophin-2) ELISA Kit (CUSTOM ELISA KIT)/ Mouse Ctf2 (Cardiotrophin-2) ELISA试剂盒 (CUSTOM ELISA试剂盒)

Mouse Ctf2 (Cardiotrophin- 2) ELISA Kit (CUSTOM ELISA KIT)

96 Tests

Operating instructions

FOR RESEARCH USE ONLY!

Alternative name

Mouse Ctf2 ELISA KIT ,Mouse Gm494 ELISA KIT ,Mouse CT-2 ELISA KIT ,Mouse neuropoietin ELISA KIT ,Mouse cardiotrophin 2 ELISA KIT

Intended use

Mouse Ctf2 ELISA kit allows for the in vitro quantitative determination of Mouse Cardiotrophin- 2 concentrations in serum ,plasma  , tissue homogenates  ,cell culture supernates , and other biological fluids.

Test principle

Mouse Ctf2 ELISA kit is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Ctf2  ,During the reaction  ,Ctf2 in the sample or standard competes with a fixed amount of biotin-labeled Ctf2 for sites on a pre-coated Monoclonal antibody specific to Ctf2. Excess conjugate and unbound sample or standard are washed from the plate. Next  ,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Ctf2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Mouse Ctf2 ELISA kit Range 0.15-10ng/ml

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Mouse Ctf2 ELISA Kit

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