ATP-dependent RNA helicase DDX25, Ddx25 ELISA KIT/ Mouse ATP-dependent RNA helicase DDX25, Ddx25 ELISA试剂盒

Product Name:Mouse ATP- dependent RNA helicase DDX25, Ddx25 ELISA KIT
Packing:96T

Catalog No.:ELI-32033m

Gene Name:Mouse Ddx25

Detect Range:0.15-10ng/ml

Sensitivity:0.094ng/ml

Target Protein Name:Mouse Ddx25

Alternative Name:Mouse ATP-dependent RNA helicase DDX25, Ddx25
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Mouse ATP- dependent RNA helicase DDX25, Ddx25 ELISA KIT allows for the in vitro quantitative determination of Mouse Ddx25 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Mouse ATP- dependent RNA helicase DDX25, Ddx25 ELISA KIT has been pre-coated with an Mouse ATP-dependent RNA helicase DDX25, Ddx25 antibody specific to Mouse Ddx25 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Ddx25 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Ddx25 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Ddx25 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!

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