Mouse Kyphoscoliosis peptidase, Ky ELISA KIT Product Name:Mouse Kyphoscoliosis peptidase, Ky ELISA KIT
Packing:96T
Catalog No.:ELI-31829m
Gene Name:Mouse Ky
Detect Range:15.62-1000pg/ml
Sensitivity:9.375pg/ml
Target Protein Name:Mouse Ky
Alternative Name:Mouse Kyphoscoliosis peptidase, Ky
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Kyphoscoliosis peptidase, Ky ELISA KIT allows for the in vitro quantitative determination of Mouse Ky concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Kyphoscoliosis peptidase, Ky ELISA KIT has been pre-coated with an Mouse Kyphoscoliosis peptidase, Ky antibody specific to Mouse Ky .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Ky and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Ky , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Ky in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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