Mouse Growth factor receptor- bound protein 10, GRB10 ELISA KIT
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
GRB10,GRBIR,GRB10 adaptor protein; GRB10 adapter protein; GRB-IR; Grb-10; IRBP; MEG1; RSS; insulin receptor-binding protein Grb-IR; maternally expressed gene 1; growth factor receptor-bound protein 10
Search name
Mouse GRB10 ELISA KIT ,Mouse GRBIR ELISA KIT ,Mouse GRB10 adaptor protein ELISA KIT ,Mouse GRB10 adapter protein ELISA KIT ,Mouse GRB-IR ELISA KIT ,Mouse Grb-10 ELISA KIT ,Mouse IRBP ELISA KIT ,Mouse MEG1 ELISA KIT ,Mouse RSS ELISA KIT ,Mouse insulin receptor-binding protein Grb-IR ELISA KIT ,Mouse maternally expressed gene 1 ELISA KIT ,Mouse growth factor receptor-bound protein 10 ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Mouse Growth factor receptor- bound protein 10, GRB10 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to GRB10. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for GRB10 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain GRB10, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of GRB10 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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