PRPF8 (Pre-mRNA-processing-splicing factor 8) ELISA Kit/ Mouse PRPF8 (Pre-mRNA-processing-splicing factor 8) ELISA试剂盒

Mouse PRPF8 (Pre- mRNA- processing- splicing factor 8) ELISA Kit (CUSTOM)

96 Tests

Operating instructions

FOR RESEARCH USE ONLY!

Alternative name

PRPF8 ELISA KIT,PRP8 ELISA KIT,PRP8 ELISA KIT,PRPC8 ELISA KIT,RP13 ELISA KIT,SNRNP220 ELISA KIT,220 kDa U5 snRNP-specific protein ELISA KIT,PRP8 homolog ELISA KIT,PRP8 pre-mRNA processing factor 8 homolog ELISA KIT,U5 snRNP-specific protein (220 kD) ELISA KIT, ortholog of S. cerevisiae Prp8p ELISA KIT,apoptosis-regulated protein 1 ELISA KIT,apoptosis-regulated protein 2 ELISA KIT,p220 ELISA KIT,precursor mRNA processing protein ELISA KIT,splicing factor Prp8 ELISA KIT,pre-mRNA processing factor 8 ELISA KIT

Intended use

Mouse PRPF8 ELISA kit allows for the in vitro quantitative determination of Mouse Pre- mRNA- processing- splicing factor 8 concentrations in serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.

Test principle

Mouse PRPF8 ELISA kit is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to PRPF8, During the reaction, PRPF8 in the sample or standard competes with a fixed amount of biotin-labeled PRPF8 for sites on a pre-coated Monoclonal antibody specific to PRPF8. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of PRPF8 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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