Mouse Tumor necrosis factor alpha- induced protein 3, TNFAIP3 ELISA KIT
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
Tumor necrosis factor alpha-induced protein 3,TNF alpha-induced protein 3,Zinc finger protein A20,OTU domain-containing protein 7C,Putative DNA-binding protein A20, TNFAIP3,A20,OTUD7C; TNFA1P2; OTU domain-containing protein 7C; TNF alpha-induced protein 3; tumor necrosis factor alpha-induced protein 3; tumor necrosis factor inducible protein A20; zinc finger protein A20; tumor necrosis factor, alpha-induced protein 3
Search name
Mouse Tumor necrosis factor alpha-induced protein 3 ELISA KIT ,Mouse TNF alpha-induced protein 3 ELISA KIT ,Mouse Zinc finger protein A20 ELISA KIT ,Mouse OTU domain-containing protein 7C ELISA KIT ,Mouse Putative DNA-binding protein A20 ELISA KIT ,Mouse TNFAIP3 ELISA KIT ,Mouse A20 ELISA KIT ,Mouse OTUD7C ELISA KIT ,Mouse TNFA1P2 ELISA KIT ,Mouse OTU domain-containing protein 7C ELISA KIT ,Mouse TNF alpha-induced protein 3 ELISA KIT ,Mouse tumor necrosis factor alpha-induced protein 3 ELISA KIT ,Mouse tumor necrosis factor inducible protein A20 ELISA KIT ,Mouse zinc finger protein A20 ELISA KIT ,Mouse tumor necrosis factor ELISA KIT ,Mouse alpha-induced protein 3 ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Mouse tumor necrosis factor alpha-induced protein 3,TNF alpha-induced protein 3 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to TNF alpha-induced protein 3. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for TNF alpha-induced protein 3 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain TNF alpha-induced protein 3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of TNF alpha-induced protein 3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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