UBE2M (NEDD8-conjugating enzyme Ubc12) ELISA Kit (CUSTOM)/ Mouse UBE2M (NEDD8-conjugating enzyme Ubc12) ELISA试剂盒 (CUSTOM)

Mouse UBE2M (NEDD8- conjugating enzyme Ubc12) ELISA Kit (CUSTOM)

96 Tests

Operating instructions

FOR RESEARCH USE ONLY!

Alternative name

Mouse UBE2M ELISA KIT ,Mouse UBC-RS2 ELISA KIT ,Mouse UBC12 ELISA KIT ,Mouse hUbc12 ELISA KIT ,Mouse NEDD8 carrier protein ELISA KIT ,Mouse NEDD8 protein ligase ELISA KIT ,Mouse ubiquitin carrier protein M ELISA KIT ,Mouse ubiquitin conjugating enzyme E2M ELISA KIT ,Mouse ubiquitin-conjugating enzyme E2M (UBC12 homolog ELISA KIT ,Mouse  yeast) ELISA KIT ,Mouse ubiquitin-protein ligase M ELISA KIT ,Mouse yeast UBC12 homolog ELISA KIT ,Mouse ubiquitin conjugating enzyme E2 M ELISA KIT

Intended use

Mouse UBE2M ELISA kit allows for the in vitro quantitative determination of Mouse NEDD8- conjugating enzyme Ubc12 concentrations in serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.

Test principle

Mouse UBE2M ELISA kit is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to UBE2M, During the reaction, UBE2M in the sample or standard competes with a fixed amount of biotin-labeled UBE2M for sites on a pre-coated Monoclonal antibody specific to UBE2M. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of UBE2M in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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