Mouse Filamin- B, FLNB ELISA KIT
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
FLNB,ABP-278; ABP-280; AOI; FH1; FLN-B; FLN1L; LRS1; SCT; TABP; TAP; ABP-280 homolog; Larsen syndrome 1 (autosomal dominant); actin binding protein 278; actin-binding-like protein; beta-filamin; filamin homolog 1; filamin-3; filamin-B; thyroid autoantigen; filamin B, beta
Search name
Mouse FLNB ELISA KIT ,Mouse ABP-278 ELISA KIT ,Mouse ABP-280 ELISA KIT ,Mouse AOI ELISA KIT ,Mouse FH1 ELISA KIT ,Mouse FLN-B ELISA KIT ,Mouse FLN1L ELISA KIT ,Mouse LRS1 ELISA KIT ,Mouse SCT ELISA KIT ,Mouse TABP ELISA KIT ,Mouse TAP ELISA KIT ,Mouse ABP-280 homolog ELISA KIT ,Mouse Larsen syndrome 1 autosomal dominant ELISA KIT ,Mouse actin binding protein 278 ELISA KIT ,Mouse actin-binding-like protein ELISA KIT ,Mouse beta-filamin ELISA KIT ,Mouse filamin homolog 1 ELISA KIT ,Mouse filamin-3 ELISA KIT ,Mouse filamin-B ELISA KIT ,Mouse thyroid autoantigen ELISA KIT ,Mouse filamin B beta ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Mouse Filamin- B, FLNB concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Filamin-B. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Filamin-B and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Filamin-B, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Filamin-B in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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