Mouse Ellis- van Creveld syndrome protein homolog, Evc ELISA KIT Product Name:Mouse Ellis- van Creveld syndrome protein homolog, Evc ELISA KIT
Packing:96T
Catalog No.:ELI-09268m
Gene Name:Mouse Evc
Detect Range:15.62-1000pg/ml
Sensitivity:9.375pg/ml
Target Protein Name:Mouse Evc
Alternative Name:Mouse Ellis-van Creveld syndrome protein homolog, Evc
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Ellis- van Creveld syndrome protein homolog, Evc ELISA KIT allows for the in vitro quantitative determination of Mouse Evc concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Ellis- van Creveld syndrome protein homolog, Evc ELISA KIT has been pre-coated with an Mouse Ellis-van Creveld syndrome protein homolog, Evc antibody specific to Mouse Evc .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Evc and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Evc , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Evc in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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