Mouse Tumor necrosis factor receptor superfamily member 12A, TNFRSF12A ELISA KIT
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
TNFRSF12A,CD266; FN14; TWEAKR; FGF-inducible 14; fibroblast growth factor-inducible immediate-early response protein 14; tweak-receptor; type I transmembrane protein Fn14; tumor necrosis factor receptor superfamily, member 12A
Search name
Mouse TNFRSF12A ELISA KIT ,Mouse CD266 ELISA KIT ,Mouse FN14 ELISA KIT ,Mouse TWEAKR ELISA KIT ,Mouse FGF-inducible 14 ELISA KIT ,Mouse fibroblast growth factor-inducible immediate-early response protein 14 ELISA KIT ,Mouse tweak-receptor ELISA KIT ,Mouse type I transmembrane protein Fn14 ELISA KIT ,Mouse tumor necrosis factor receptor superfamily member 12A ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of mouse tumor necrosis factor receptor superfamily member 12a,TNFRSF12A concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to TNFRSF12A. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for TNFRSF12A and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain TNFRSF12A, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of TNFRSF12A in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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