Glycogen synthase kinase-3 beta, Gsk3b ELISA KIT/ Mouse Glycogen synthase kinase-3 beta, Gsk3b ELISA试剂盒

Mouse Glycogen synthase kinase-3 beta,GSK3B ELISA Kit

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

2.7.11.26,Glycogen synthase kinase-3 beta,GSK-3 beta,2.7.11.1,Serine/threonine-protein kinase GSK3B,GSK3B,GSK3betaserine/threonine-protein kinase GSK3B, GSK-3 beta, GSK3beta isoform, glycogen synthase kinase-3 beta, glycogen synthase kinase 3 beta

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Mouse 2.7.11.26 ELISA KIT ,Mouse Glycogen synthase kinase-3 beta ELISA KIT ,Mouse GSK-3 beta ELISA KIT ,Mouse 2.7.11.1 ELISA KIT ,Mouse Serine/threonine-protein kinase GSK3B ELISA KIT ,Mouse GSK3B ELISA KIT ,Mouse GSK3betaserine/threonine-protein kinase GSK3B ELISA KIT ,Mouse GSK-3 beta ELISA KIT ,Mouse GSK3beta isoform ELISA KIT ,Mouse glycogen synthase kinase-3 beta ELISA KIT ,Mouse glycogen synthase kinase 3 beta ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination of Mouse glycogen synthase kinase-3 beta,GSK-3 beta concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to GSK-3 beta. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for GSK-3 beta and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain GSK-3 beta, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of GSK-3 beta in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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