Cytochrome P450 19A1, Cyp19a1 ELISA KIT/ Mouse Cytochrome P450 19A1, Cyp19a1 ELISA试剂盒

Mouse Cytochrome P450 19A1, CYP19A1 ELISA KIT

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

Cyp19a1,aromatase,ARO; ARO1; CPV1; CYAR; CYP19; CYPXIX; P-450AROM; cytochrome P-450AROM; cytochrome P450 19A1; cytochrome P450, subfamily XIX (aromatization of androgens); estrogen synthase; estrogen synthetase; flavoprotein-linked monooxygenase; microsomal monooxygenase; cytochrome P450 family 19 subfamily A polypeptide 1

Search name

Mouse Cyp19a1 ELISA KIT ,Mouse aromatase ELISA KIT ,Mouse ARO ELISA KIT ,Mouse ARO1 ELISA KIT ,Mouse CPV1 ELISA KIT ,Mouse CYAR ELISA KIT ,Mouse CYP19 ELISA KIT ,Mouse CYPXIX ELISA KIT ,Mouse P-450AROM ELISA KIT ,Mouse cytochrome P-450AROM ELISA KIT ,Mouse cytochrome P450 19A1 ELISA KIT ,Mouse cytochrome P450 ELISA KIT ,Mouse  subfamily XIX (aromatization of androgens) ELISA KIT ,Mouse estrogen synthase ELISA KIT ,Mouse estrogen synthetase ELISA KIT ,Mouse flavoprotein-linked monooxygenase ELISA KIT ,Mouse microsomal monooxygenase ELISA KIT ,Mouse cytochrome P450 family 19 subfamily A polypeptide 1 ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination of mouse Cytochrome P450 19A1 concentrations in serum, Plasma, Urine, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cytochrome P450 19A1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Cytochrome P450 19A1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Cytochrome P450 19A1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Cytochrome P450 19A1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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